Neglected tropical diseases

Insecticide resistance

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Discriminating concentrations

Test guidelines

Resistance monitoring should be an integral part of vector/public health pest control programmes. Knowledge of vector/pest susceptibility to pesticides, changing trends of resistance and their operational implications are basic requirements to guide pesticide use in vector-borne disease and pest control programmes.

This information provides a basis for selecting pesticide(s), for ascertaining continued susceptibility to and efficacy of pesticide(s) in use, and for vector/pest pesticide resistance management.

Test procedures for insecticide resistance monitoring in malaria vector mosquitoes

This second edition provides an overview of two new methods for measuring insecticide resistance (IR): the intensity bioassay and the synergist bioassay. These new methods use the current WHO susceptibility test to measure the intensity of insecticide resistance and to determine one of the main underlying metabolic mechanisms. Data generated by these tests will enable countries to track the evolution of insecticide resistance, inform the development of national insecticide resistance management strategies, and ensure the effective use of available tools.

Insecticide resistance monitoring

Test procedures for insecticide resistance monitoring in malaria vectors, bio-efficacy and persistence of insecticide on treated surfaces, Geneva, World Health Organization, 1998. Ref: WHO/CDS/CPC/MAL/98.12

Discriminating concentrations of insecticides for adult mosquitoes

The correct use of biochemical or molecular methods for resistance detection at a mechanistic level can provide a powerful tool for analysing vector/pest populations with the aim of improving resistance detection and management.

Techniques to detect insecticide resistance mechanisms (field and laboratory manual), Geneva, World Health Organization. Ref: WHO/CDS/CPC/MAL/98.6

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